Tosyl phenylalanyl chloromethyl ketone Totally Explained

Everything about Tosyl Phenylalanyl Chloromethyl Ketone totally explained

| Section2 = | Section3 = }} Tosyl phenylalanyl chloromethyl ketone (TPCK) is a protease inhibitor. Its structural formula is 1-chloro-3-tosylamido-4-phenyl-2-butanone.

Uses

TPCK is the irreversible inhibitor of chymotrypsin. Also inhibits some cysteine proteases such as papain, bromelain or ficin. It doesn't inhibit trypsin nor zymogens.
TPCK is chosen for the chemical labelling of active histidine in enzyme analysis. The phenylalanine moiety is bound to the enzyme because of specificity for aromatic amino acid residues at the active site (as chymotrypsin).

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